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KMID : 0370220140580040223
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Yakhak Hoeji
2014 Volume.58 No. 4 p.223 ~ p.228
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The Inhibitory Effect of Broccoli in Cruciferous Vegetables Derived-Sulforaphane on Vascular Tension
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Je Hyun-Dong
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Abstract
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The present study was undertaken to investigate the influence of sulforaphane on vascular smooth muscle contractility and to determine the mechanism involved. We hypothesized that sulforaphane, the primary ingredient of broccoli of cruciferous vegetables, plays a role in vascular relaxation through inhibition of Rho-kinase in rat aortae. Intact of denuded arterial rings from male Sprague-Dawley rats were used and isometric tensions were recorded using a computerized data acquisition system. Interestingly, sulforaphane significantly inhibited fluoride, phorbol ester or thromboxane A2 mimeticinduced contraction in denuded muscles suggesting that additional pathways different from endothelial nitric oxide synthesis such as inhibition of Rho-kinase or MEK might be involved in the vasorelaxation. Furthermore, sulforaphane inhibited thromboxane A2-induced increases in pERK1/2 levels suggesting the mechanism including inhibition of thromboxane A2-
induced increases in ERK1/2 phosphorylation. This study provides evidence that sulforaphane induces vascular relaxation
through inhibition of Rho-kinase or MEK in rat aortae.
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KEYWORD
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endothelium, fluoride, MEK, phorbol ester, Rho-kinase, sulforaphane, vasoconstriction
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